antibodies against pzp  (Proteintech)

Journal: BMJ Neurology Open

Article Title: Altered amyloid plasma profile in patients with disabling headaches after SARS-CoV-2 infection and vaccination

doi: 10.1136/bmjno-2024-001013

Figure Lengend Snippet: Plasma levels of (A) amyloid precursor protein (APP), (B) cathepsin L, (C) pregnancy zone protein (PZP) and (D) serum amyloid A (SAA1) in healthy controls (HC) (n=16), participants with persistent headache after SARS-CoV-2 vaccine (COvax) (n=31) and participants with persistent headache after COVID-19 ( C19 ) (n=29).

Article Snippet: Plasma levels of APP (Cat# DY850), PZP (Cat# DY8280-05), CTSL (Cat# DY952) and SAA1 (Cat# DY3019-05) were measured by ELISA using commercially available antibodies (R&D Systems, Minneapolis, Minnesota, USA) in a 384-format using a combination of a SELMA pipetting robot (Analytik Jena AG, Jena, Germany) and a BioTek dispenser/washer (BioTek Instruments, Winooski, VT).

Techniques: Clinical Proteomics

Journal: Stem cells translational medicine

Article Title: Mesenchymal stromal cells-derived small extracellular vesicles protect against UV-induced photoaging via regulating pregnancy zone protein.

doi: 10.1093/stcltm/szae069

Figure Lengend Snippet: Figure 5. Screening and verification of the differentially expressed gene pzp in DFs and skins based on RNA-seq. (A) DEGs thermograms of 3 independent duplicate samples of the Ctrl, UV, and UV + sEV groups. (B, C) Volcano maps (B) and Venn maps (C) of DEGs. (D) R language analysis of TOP DEGs. (E) RNA-seq analysis of DEGs, including pzp, sall1, fmo2, trh, and tex26. (F) RT-qPCR validation of the DEGs. (G) Western blot detection of the expression of PZP, FMO2, and γ-H2AX. (H) GO functional analysis of pzp. (I) Immunohistochemical detection of PZP expression in skin tissues of nude mice on day 35 of modeling. Scale bar = 20 μm. Data are presented as mean ± SD. n = 3, **P < .01, ***P < .001, ns, no significant difference.

Article Snippet: The primary antibodies are listed below: β-actin (1:5000, Abclonal), COL1A1 (1:500, Abclonal), MMP-1 (1:500, Abclonal), SIRT3 (1:500, Wanleibio), γ-H2AX (1:500, CST), PZP (1:500, Proteintech), ataxia telangiectasia-mutated protein (ATM, 1:500, Abclonal), p-ATM (1:500, Abclonal), 53BP1 (1:500, Proteintech), MMP-9 (1:500, Wanleibio), FMO2 (1:500, Proteintech), Alix (1:500, CST), CD9 (1:500, CST), CD81 (1:500, Proteintech), CD63 (1:500, Proteintech), TSG101 (1:500, Proteintech), and Calnexin (1:500, CST).

Techniques: RNA Sequencing, Quantitative RT-PCR, Biomarker Discovery, Western Blot, Expressing, Functional Assay, Immunohistochemical staining

Journal: Stem cells translational medicine

Article Title: Mesenchymal stromal cells-derived small extracellular vesicles protect against UV-induced photoaging via regulating pregnancy zone protein.

doi: 10.1093/stcltm/szae069

Figure Lengend Snippet: Figure 6. Validation of PZP and MMP-1 interaction. (A) Known proteins interacting with PZP identified by the STRING database. (B) Predicted protein docking pattern of PZP and MMP-9. (C) Predicted protein docking pattern of PZP and MMP-1. (D) Validation of the interaction between PZP and MMP-1 via co-immunoprecipitation. (E) The co-localization of PZP and MMP-1 in DFs was assessed using immunofluorescence assay. Scale bar = 20 μm. (F) Immunofluorescence co-localization analysis of PZP and MMP-1 in skin tissues of nude mice. Scale bar = 200 μm.

Article Snippet: The primary antibodies are listed below: β-actin (1:5000, Abclonal), COL1A1 (1:500, Abclonal), MMP-1 (1:500, Abclonal), SIRT3 (1:500, Wanleibio), γ-H2AX (1:500, CST), PZP (1:500, Proteintech), ataxia telangiectasia-mutated protein (ATM, 1:500, Abclonal), p-ATM (1:500, Abclonal), 53BP1 (1:500, Proteintech), MMP-9 (1:500, Wanleibio), FMO2 (1:500, Proteintech), Alix (1:500, CST), CD9 (1:500, CST), CD81 (1:500, Proteintech), CD63 (1:500, Proteintech), TSG101 (1:500, Proteintech), and Calnexin (1:500, CST).

Techniques: Biomarker Discovery, Immunoprecipitation, Immunofluorescence

Journal: Stem cells translational medicine

Article Title: Mesenchymal stromal cells-derived small extracellular vesicles protect against UV-induced photoaging via regulating pregnancy zone protein.

doi: 10.1093/stcltm/szae069

Figure Lengend Snippet: Figure 7. PZP ameliorates photoaging by mainly inhibiting MMP-1 expression. After overexpression of the pzp gene (A) SA β-gal staining and its quantization diagram. Scale bar = 100 μm. (B) Detection of the expression of PZP, COL1A1, MMP-1, SIRT3, and ATM signaling pathway-related proteins by Western blot. (C) Fluorescence images of mitotracker staining and SIRT3. Scale bar = 200 μm. (D) Immunofluorescence detection of γ-H2AX expression. Scale bar = 200 μm. After knockdown of the pzp gene (E) SA β-gal staining and its quantization diagram. Scale bar = 100 μm. (F) The levels of COL1A1, MMP-1, and DDR-related proteins were quantified by Western blot. (G) Fluorescence images of mitotracker staining and SIRT3. Scale bar = 200 μm. (H) Analysis of γ-H2AX expression by immunofluorescence. Scale bar = 200 μm. Ctrl + vector: DFs transfected with empty vector,

Article Snippet: The primary antibodies are listed below: β-actin (1:5000, Abclonal), COL1A1 (1:500, Abclonal), MMP-1 (1:500, Abclonal), SIRT3 (1:500, Wanleibio), γ-H2AX (1:500, CST), PZP (1:500, Proteintech), ataxia telangiectasia-mutated protein (ATM, 1:500, Abclonal), p-ATM (1:500, Abclonal), 53BP1 (1:500, Proteintech), MMP-9 (1:500, Wanleibio), FMO2 (1:500, Proteintech), Alix (1:500, CST), CD9 (1:500, CST), CD81 (1:500, Proteintech), CD63 (1:500, Proteintech), TSG101 (1:500, Proteintech), and Calnexin (1:500, CST).

Techniques: Expressing, Over Expression, Staining, Western Blot, Fluorescence, Immunofluorescence, Knockdown, Plasmid Preparation, Transfection

Journal: The World Allergy Organization Journal

Article Title: Serum proteomics identify CSF1R as a novel biomarker for postoperative recurrence in chronic rhinosinusitis with nasal polyps

doi: 10.1016/j.waojou.2024.100878

Figure Lengend Snippet: Validating serum concentrations of the potential DEPs and exploring the predictive values for CRSwNP recurrence in the validation cohort. (A) CSF1R; (B) CDC42; (C) CCL5; (D) UBE2V1; (E) PZP; (F) DHRS9. (G–I) ROC curves; (J–L) Kaplan-Meier survival analysis. ROC, receiver operator characteristic; CRSwNP, chronic rhinosinusitis with nasal polyps. ∗P < 0.05; ∗∗∗P < 0.001; ns, no significance

Article Snippet: Cell division control protein 42 (CDC42) ELISA kits, ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1) ELISA kits, pregnancy zone protein (PZP) ELISA kits, dehydrogenase/reductase (DHRS9) ELISA kits were provided by ARP Inc™ (Waltham, USA).

Techniques:

Journal: Frontiers in immunology

Article Title: TMT-Based Quantitative Proteomics Analysis of Synovial Fluid-Derived Exosomes in Inflammatory Arthritis.

doi: 10.3389/fimmu.2022.800902

Figure Lengend Snippet: FIGURE 7 | Verification of exosome-derived PZP in SF by ELISA. The ELISA kits were performed to detect the PZP level of SF-derived exosomes in gout, axSpA, OA, and RA. *P value < 0.05 vs. OA groups.

Article Snippet: The ELISA Kits (CSBEL019131HU, CUSABIO BIOTECH) were performed to detect the pregnancy zone protein (PZP) level of SF-derived exosomes in all groups, following the manufacturer’s instructions.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Life

Article Title: LC-MS/MS-Based Serum Protein Profiling for Identification of Candidate Biomarkers in Pakistani Rheumatoid Arthritis Patients

doi: 10.3390/life12030464

Figure Lengend Snippet: The serum levels of human PZP proteins quantified using ELISA in RA patients ( n = 60) and healthy controls ( n = 20). t -test was used to compare the serum concentration of PZP. Boxplot was designed using R 4.1.1.

Article Snippet: A human PZP ELISA kit (CSB-EL019131HU, CUSABIO, Houston, TX, USA) was used for the quantification of PZP protein in human samples from an independent cohort of RA patients and controls according to the manufacturer’s directions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay